University of Iowa
Department of Biochemistry
4-403 BSB
Iowa City, IA 52242-1109 USA phone: 877-846-8569
or 319-335-7932
fax: (319) 335-9570
biochem@uiowa.edu

Department of Biochemistry

Recent Publications :: Bryce Plapp

Kovaleva, E.G., and Plapp, B.V. (2007) Deprotonation of the horse liver alcohol dehydrogenase-NAD+ complex controls formation of the ternary complexes. Biochemistry 27:12797-12808.
Plapp, B.V. and Berst, K.B. (2006) Human alcohol dehydrogenase 4: Mechanism, specificity and effects of ethanol on retinoid metabolism in Enzymology and Molecular Biology of Carbonyl Metabolism - 12. Henry Weiner, Bryce V. Plapp, Ronald Lindahl, and Edmund Maser, Eds., University Press, West Lafayette, IN, pp. 190-199.
Plapp, B.V., and Berst, K.B. (2005) Human alcohol dehydrogenase 4: Mechanism, specificity, and effects of ethanol on retinoid metabolism, in H. Weiner (Ed.) Enzymology and Molecular Biology of Carbonyl Metabolism: Aldehyde Dehydrogenase, Aldo-Keto Reductase and Alcohol Dehydrogenase, Purdue University Press, pp. 187-196.
Catalysis by alcohol dehydrogenases. Bryce V. Plapp, in Isotope effects in chemistry and biology (Amnon Kohen and Hans-Heinrich Limbach, eds.), CRC Press 2005, pp. 811-835.
Kovaleva, E.G., and Plapp, B.V. (2005) Deprotonation of the horse liver alcohol dehydrogenase-NAD+ complex controls formation of the ternary complexes. Biochemistry 44:12797-12808.
Collins, X.H., Harmon, S.D., Kaduce, T.L., Berst, K.B., Fang, X., Moore, S.A., Raju, T.V., Falck, J.R., Weintraub, N.L., Duester, G., Plapp, B.V., and Spector, A.A. (2005) w-Oxidation of 20-hydroxyeicosatetraenoic Acid (20-HETE) in cerebral microvascular smooth muscle and endothelium by alcohol dehydrogenase 4. J. Biol. Chem. 280:33157-33164.
LeBrun, L.A., Park, D.-H., Ramaswamy, S., and Plapp, B.V. (2004) Participation of histidine-51 in catalysis by horse liver alcohol dehydrogenase. Biochemistry 43: 3014-3026.
Strömberg, P., Svensson, S., Berst, K.B., Plapp, B.V., and Höög, J.-O. (2004) Enzymatic mechanism of low-activity mouse alcohol dehydrogenase 2. Biochemistry 43:1323-1328.
Venkataramaiah, T.H. and Plapp, B.V. (2003) Formamides mimic aldehydes and inhibit liver alcohol dehydrogenases and ethanol metabolism. J. Biol. Chem. 278:36699-36706.
Plapp, B.V., and Berst, K.B., (2003) Specificity of human alcohol dehydrogenase 1C*2 (g2g2) for steroids and simulation of the uncompetitive inhibition of ethanol metabolism. Chem. Biol. Interact. 143-144:183-193.
Rubach, J.K. and Plapp, B.V. (2003) Amino acid residues in the nicotinamide binding site contribute to catalysis by horse liver alcohol dehydrogenase. Biochemistry 42:2907-2915.
Rubach, J.K. and Plapp, B.V. (2002) Mobility of fluorobenzyl alcohols bound to liver alcohol dehydrogenases as determined by NMR and X-ray crystallographic studies. Biochemistry 41:15770-15779.
Rubach, J.K., Ramaswamy, S., and Plapp, B.V. (2001) Contributions of valine-292 in the nicotinamide binding site of liver alcohol dehydrogenase and dynamics to catalysis. Biochemistry 40:12686-12694.
Plapp, B.V., Mitchell, J.L., and Berst, K.B. (2001) Mouse alcohol dehydrogenase 4: Kinetic mechanism, substrate specificity and simulation of effects of ethanol on retinoid metabolism. Chem-Biol Interact. 130-132:445-456.

Figure 1. Structure of horse liver alcohol dehydrogenase complexed with substrates (Ramaswamy et al., 1994)

Figure 2. Evolutionary tree prepared by Jeniffer L. Mitchell March 24, 1998

Download kinetics programs for PCs operating under DOS or Windows: Cleland’s programs for fitting and graphing steady-state enzyme kinetic data. Frieden’s KINSIM and FITSIM programs for simulating progress curves. Read the .DOC or .TXT files for information on uncompressing and installing the programs. Included are sample input files for these programs: e.g., .STD files for Cleland’s programs and .MEC, .SIM, .SAV, .RDF, and .FDT Frieden’s programs. Download Kin Dist [.zip format].

Also see PubMed for recent publications.