Recent Publications from the Donelson Lab.

Tetracycline-regulated RNA interference in Trypanosoma congolense.
Inoue N, Otsu K, Ferraro DM, Donelson JE., Mol Biochem Parasitol. 2002 Apr 9;120(2):309-313.

Trypanosoma brucei FLA1 is required for flagellum attachment and cytokinesis.
LaCount DJ, Barrett B, Donelson JE., J Biol Chem. 2002 Mar 4

Trypanin is a cytoskeletal linker protein and is required for cell motility in African trypanosomes.
Hutchings NR, Donelson JE, Hill KL., J Cell Biol. 2002 Mar 4;156(5):867-877

Comparison of the post-transcriptional regulation of the mRNAs for the surface proteins PSA (GP46) and MSP (GP63) of Leishmania chagasi., Myung KS, Beetham JK, Wilson ME, Donelson JE., J Biol Chem. 2002 Feb 20

RNA interference in African trypanosomes., LaCount DJ, Donelson JE., Protist. 2001 Jul;152(2):103-111.

Analysis of a donor gene region for a variant surface glycoprotein and its expression site in African trypanosomes., LaCount DJ, El-Sayed NM, Kaul S, Wanless D, Turner CM, Donelson JE., Nucleic Acids Res. 2001 May 15;29(10):2012-2019

Cell cycle expression of histone genes in Trypanosoma cruzi., Recinos RF, Kirchhoff LV, Donelson JE., Mol Biochem Parasitol. 2001 Apr 6;113(2):215-222

Conservation of metacyclic variant surface glycoprotein expression sites among different trypanosome isolates., Bringaud F, Biteau N, Donelson JE, Baltz T., Mol Biochem Parasitol. 2001 Mar;113(1):67-78

Regulation of GP63 mRNA stability in promastigotes of virulent and attenuated Leishmania chagasi.
Brittingham A, Miller MA, Donelson JE, Wilson ME., Mol Biochem Parasitol. 2001 Jan 15;112(1):51-59

Hill, Kent L., Hutchings, Nathan R., Grandgenett, Paul M., and Donelson, John E. (2000). T-lymphocyte Triggering Factor of African Trypanosomes is Associated with the Flagellar fraction of the Cytoskeleton and Represents a new Family of Proteins that are present in several Divergent Eukaryotes. Journal of Biological Chemistry. 275 (50) : 39369 -39378.  Abstract.

LaCount, Doug L., Bruse, Shannon, Hill, Kent L., and Donelson, John E. (2000) Double-stranded RNA interference in trypanosoma brucei using head-to-head promoters. Molecular and  Biochemical Parasitol. Nov;111(1):67-76.  Abstract.

Grandgenett, P.M., Coughlin, B.C., Kirchhoff, L.V., and Donelson, J.E. (2000). Differential Expression of GP63 Genes in Trypanosoma cruzi. Mol. Biochem. Parasitol. (in press). Abstract.

Coughlin, B.C., Teixeira, S., Kirchhoff, L.V., and Donelson, J.E. (2000). Amastin mRNA abundance in Trypanosoma cruzi is controlled by a 3’ untranslated region position dependent cis-element and an untranslated region binding protein. J. Biol. Chem. 275: 12051-12060. Abstract.

El-Sayed, N.M, Hegde, P., Quackenbush, J., Melville, S.E., and Donelson, J.E. (2000). The African trypanosome genome. Intern. J. Parasitol. 30:329-345. Abstract.

Downey, N., and Donelson, J.E. (1999). Expression of foreign proteins in Trypanosoma congolense. Mol. Biochem. Parasitol. 104:39-53. Abstract.

Downey, N., and Donelson, J.E. (1999). Search for promoters for the GARP and rRNA genes of Trypanosoma congolense. Mol. Biochem. Parasitol. 104:25-38. Abstract.

Donelson, J.E., Hill, K.L., and El-Sayed, N.M.A. (1999). Multiple mechanisms of immune evasion by Trypanosoma brucei. In The Trypanosome Surface (Pays, E., Ed.) Pub.: De Boeck & Larcier, Brussels, Belgium, pp. 71-91.

Donelson, J.E., Gardner, M.J., and El-Sayed, N.M. (1999). More surprises from Kinetoplastidae. Proc. Natl. Acad. Sci. U.S.A. 96:2579-2581. 

Hill, K.L., Hutchings, N.R., Russell, D.G., and Donelson, J.E. (1999). A novel protein targeting domain confers flagellar pocket localization in African trypanosomes. J. Cell Sci. 112:3091-3101. Abstract. Download reprint.

Pépin, J., and Donelson, J.E. (1999). Human Trypanosomiasis (Sleeping Sickness). In Tropical Infectious Diseases: Principles, Pathogens & Practice (Guerrant, R.L., Walker, D.H., and Weller, P.F., Eds.) Pub: Churchill Livingstone. pages 774-784.

Catmull, J., Wilson, M.E., Kirchhoff, L.V., Metwali, A., and Donelson, J.E. (1999). Induction of specific cell-mediated immunity in mice by oral immunization with Salmonella expressing Onchocerca volvulus glutathione S-transferase. Vaccine 17:31-39.

Teixeira, S.M.R., Otsu, K., Hill, K.L., Kirchhoff, L.V., and Donelson, J.E. (1999). Expression of a marker for intracellular Trypanosoma cruzi amastigotes in extracellular spheromastigotes. Mol. Biochem. Parasitol. 98:265-270.

Teixeira, S.M.R., Kirchhoff, L.V., and Donelson, J.E. (1999). Trypanosoma cruzi: suppression of tuzin gene expression by its 5’-UTR and spliced leader addition site. Exptl. Parasitol. 93:143-151. Abstract.

Alarcon, C.M., Pedram, M., and Donelson, J.E. (1999). Leaky transcription from telomere-linked metacyclic VSG genes in African trypanosomes. J. Biol. Chem. 274:16884-16893. Abstract.

Pedram, M., and Donelson, J.E. (1999). Characterization of the bloodstream expression site of the MVAT4 VSG gene of African trypanosomes. J. Biol. Chem. 274:16876-16883. Abstract.

Donelson, J.E., and Artama, W.T. (1998). Diagnosis of Trypanosoma evansi by the polymerase chain reaction (PCR). J. Protozool. Res. 8:204-213.

Djikeng, A., Agufa, C., Donelson, J.E., and Majiwa, P.A.O. (1998). Generation of expressed sequence tags as physical landmarks in the genome of Trypanosoma brucei. Gene 221:93-106.

McCoy, J.J., Beetham, J.K., Ochs, D.E., Donelson, J.E., and Wilson, M.E. (1998). Regulatory sequences and a novel gene in the msp (gp63) gene cluster of Leishmania chagasi. Mol. Biochem. Parasitol. 95:251-265.

Melville, S., Donelson, J.E., and Majiwa, P.A. (1998). Resources available in the African trypanosome genome network. Parasitology Today 14:3-5.

Donelson, J.E., Hill, K.L., and El-Sayed, N.M.A. (1998). Multiple mechanisms of immune evasion by African trypanosomes. Mol. Biochem. Parasitol. 91: 51-66.

Majiwa, P.A.O., Djiken, A., Donelson, J.E., and Agufa, C. (1998). Contribution of genome analysis to understanding the biology of, and diseases caused by, African trypanosomes. J. Protozool Res. 8:214-223.

El-Sayed, N.M.A., and Donelson, J.E. (1997). African trypanosomes have differentially expressed genes encoding homologues of the Leishmania GP63 surface protease. J. Biol. Chem. 272:26742-26748.

Vaidya, T., Hill, K., Bakhiet, M., Hill, K., Olsson, T., Kristensson, K., and Donelson, J.E. (1997). The gene for a T-lymphocyte triggering factor from African trypanosomes. J. Exptl. Med. 186:433-438.

El-Sayed, N.M.A., Alarcon, C.M., and Donelson, J.E. (1997). New insights on African trypanosomes as revealed by their expressed sequence tags (ESTs). In Trypanosomiasis and Leishmaniasis: integrated approaches to control. (ed., G. Hide). Publishers: CAB International and the British Society for Parasitology. pp 51-63.

El-Sayed, N.M.A., and Donelson, J.E. (1997). A survey of the Trypanosoma brucei rhodesiense genome using shotgun sequencing. Mo. Biochem. Parasitol. 84:167-178.

Beetham, J.K., Myung, K.S., McCoy, J.J., Wilson, M.E., and Donelson, J.E. (1997). GP46 mRNA abundance is post-transcriptionally regulated during development of Leishmania chagasi to an infectious form. J. Biol. Chem. 272:17360-17366.

Kim, K.S., and Donelson, J.E. (1997). Co-duplication of a VSG gene and its promoter to an expression site in African trypanosomes. J. Biol. Chem. 272:24637-24645.

Li, S., and Donelson, J.E. (1996). Leishmania chagasi: a gene encoding a protein kinase with a catalytic domain structurally related to MAPKK. Exptl. Parasitol. 82:87-96.

Streit, J.A., Donelson, J.E., Agey, M.W., and Wilson, M.E. (1996) Developmental changes in the expression of Leishmania chagasi gp63 and heat shock proteins in a human macrophage cell line. Infect. & Immunity 64:1810-1818.

Gay, L., Wilson, M.E., and Donelson, J.E. (1996). The promoter for the rRNA genes of Leishmania chagasi:. Mol. Biochem. Parasitol. 77:193-200.

Ramamoorthy, R., Donelson, J.E., and Wilson, M.E. (1996). 5' sequences essential for trans-splicing of msp (gp63) RNAs in Leishmania chagasi. Mol. Biochem. Parasitol. 77:65-76.

Donelson, J.E. (1996). Genome research and evolution in trypanosomes. Current Opinion in Genetics & Development 6:699-703.

Morgan, R.W., El-Sayed, N.M.A., Kepa, J.K., Pedram, M., and Donelson, J.E. (1996). Differential expression of the ESAG-I family in African trypanosomes. J. Biol Chem. 271:9771-9777.