Click here for some brief instructions.

A B C D E F G H I J K L M N O P Q R S T U V W X Y Z

A.

Acetate NaCl buffer                                         ( .htm)

Acrylamide                                                         ( .htm)

Affinity purification of antibodies                 ( .htm)

Alkaline carbonate buffer                                ( .htm)

Ammonium acetate                                          ( .htm)

B.

Blunt ending vectors                                        ( .htm)

Blunting a restriction digest                           ( .htm)

Blunting a PCR fragment                               ( .htm)

Blue/white screening                                      ( .htm)

C.

Calcium chloride                                               ( .htm)

cDNA Library Screening (Filter washings)  ( .htm)

CHO cells

        cell maintenance                                          ( .htm)

        transfection                                                  ( .htm)

Cloning blunt ends                                              ( .htm)

CNBr Sepharose Handbook                             (Amershamm-Pharmacia, *.pdf)

Continuous semidry transfer                            ( .htm)

Coomassie stain                                                  ( .htm)

Cos-7 cells 

           IFAs                                                             ( .htm)

          cell maintenance                                        ( .htm)

          RNA prep                                                    ( .htm)

Counting trypanosomes                                      ( .htm)

Coupling Antigen to CNBr sepharose              ( .htm)

Cytomix                                                                  ( .htm)

Cytoskeleton

        immunofluorescence assay                          ( .htm)

        Cytoskeleton Prep (trypanosomes)            ( .htm)

D.

DMSO stocks of Trypanosomes                          ( .htm)

DNA

        loading buffer 10X                                         ( .htm)

        maxi prep                                                         ( .htm)

        miniprep                                                          ( .htm)

vaccination of mice                                       ( .htm)

Gutat10.1 cDNA library                                      ( .htm)

Drug cocktail for triple selection                          ( .htm)

E.

E. Coli transformation                                          ( .htm)

EDTA (0.5 M)                                                         ( .htm)

Ethidium Bromide staining trypanosomes      ( .htm)

F.

Fetal calf serum                                                      ( .htm)

Freeze-squeeze DNA isolation                            ( .htm)

Frozen stocks of trypanosomes

        DMSO                                                             ( .htm)

        Glycerol                                                          ( .htm)

G.

Gel Mobility Shift Assay                                      ( .htm)

Genomic DNA from Tryps                                  ( .htm)

Gluteraldehyde (0.5%)                                          ( .htm)

Glycine

        100 mM                                                          ( .htm)

        for quenching PFA                                      ( .htm)

         pH8 in Coupling buffer                              ( .htm)

Grunstein Transfers                                              ( .htm)

H.

Heat inactivating fetal calf serum                      ( .htm)

HEPES.KOH (5 mM)                                            ( .htm)

HMI-9 media for BSF T. brucei

Hypotonic lysis buffer (J. Bangs)                        ( .htm)

I.

Imidizole dialyzing buffer                                  ( .htm)

Immunofluorescence Assay

        Immunofluorescence assay                          ( .htm)

        solutions                                                          ( .htm)

        on isolated flagella                                          ( .htm)

on isolated cytoskeletons                              ( .htm)

J.

K.

L.

Lab Saftey Instructions

Laemmli buffers for SDS_PAGE                          ( .htm)

LB media                                                                  ( .htm)

LDNT media for T. cruzi culturing

Lower SDS_PAGE Gels (Resolving)                    ( .htm)

Lysis buffer (5X) (J.Bangs)                                     ( .htm)

M.

Magnesium Acetate                                                ( .htm)

Magnesium Chloride                                              ( .htm)

Manganese Chloride                                              ( .htm)

MSMS Saftey Data Sheets

Microscope Sign Up Sheet                                    (Excel worksheet)

MME (cytoskeleton buffer)                                   ( .htm)

Mouse Bleeds

    retro-orbital mouse bleed                                  ( .htm)

    terminal mouse bleed                                         ( .htm)

N.

Nickle Column buffers                                            ( .htm)

Northern blot protocol                                            ( .htm)

NZY Top Agar                                                          ( .htm)

O.

P.

Paraformaldehyde (2%)                                          ( .htm)

PBS (phosphate buffered saline)                          ( .htm)

PCR colony lysis buffer                                           ( .htm)

PEME                                                                          ( .htm)

PMN (cytoskeleton)                                                  ( .htm)

Poly-acrylamide                                                        ( .htm)

Polymerase chain reaction                                      ( .htm)

Poly-l-lysine cover slips                                          ( .htm)

Potassium chloride                                                  ( .htm)

Potassium phosphate stock                                    ( .htm)

Procyclic trypanosome methods                            ( .htm)

Protein Purification Handbook                             (Amersham-Pharmacia, *.pdf)

PS (phosphate buffered sucrose)                          ( .htm)

Q.

Qiagen buffer P1                                                     ( .htm)

Qiagen buffer P2                                                      ( .htm)

Qiagen buffer P3                                                      ( .htm)

Qiaquick gel purification                                        ( .htm)

R.

Rapid silver stain for SDS-PAGE                          ( .htm)

Recombinant Protein Handbook                         (Amersham-Pharmacia, *.pdf)

Restriction endonuclease digestion                      ( .htm)

RNA prep from Cos-7 cells                                    ( .htm)

RPMI media for mammalian cell culture

S. 

SDM-79 (PCF T. brucei medium)                        ( .htm)

SDS-PAGE

      buffers (Laemmli)                                              ( .htm)

      gels for Western                                                 ( .htm)

Semi-dry protein transfer                                       ( .htm)

SeqWeb Analysis                                                    

Shrimp Alkaline Phosphate buffer                        ( .htm)

SM medium (Cunningham’s)                                ( .htm)

Sodium acetate                                                         ( .htm)

Sodium bicarbonate buffer                                    ( .htm)

Sodium chloride                                                      ( .htm)

Sodium Phosphate (0.1M)                                        ( .htm)

Southern Blot protocol                                              ( .htm)

T.

T4 blunting a restriction digest                              ( .htm)

T4 blunting a PCR fragment                                  ( .htm)

TAE (50X)                                                                   ( .htm)

TBE (5X)                                                                      ( .htm)

TBS (10X)                                                                   ( .htm)

T. brucei

        Cytoskeleton Prep                                             ( .htm)

        Drugs for stable trypanosome selection        ( .htm)

        DMSO stocks                                                     ( .htm)

        Electroporation media                                     ( .htm)

        Glycerol stocks                                                  ( .htm)

        Isolating genomic DNA from                          ( .htm)

        Procyclic trypanosome methods                      ( .htm)

        SM medium (Cunningham’s)                          ( .htm)

        transient transfection                                         ( .htm)

T. cruzi

        Culturing                                                            ( .htm)

        Transfection (amastigotes)                              ( .htm)

TCA precipitation of protein                                  ( .htm)

TE                                                                                  ( .htm)

Terminal mouse bleed                                              ( .htm)

Tissue Culture plate conversion / specs              ( .htm)

TRIS                                                                              ( .htm)

Triton phosphate buffer for protein                      ( .htm)

Triton X-100                                                                ( .htm)

Trypanosome transient transfection                      ( .htm)

Trypanosome frozen stocks                                     (see Frozen stocks of trypanosomes)

U.

Upper SDS-PAGE gel (stacking gel)                        ( .htm)

V.

W.

Western Blot

        Basic protocol                                                       ( .htm)

        wet transfer buffer                                               ( .htm)

X.

Y.

YEPD                                                                              ( .htm)

Z.