cDNA library from Treu 10.1
In lambda-unizap (Stratagene)
4 x 1010 pfu/ml
average insert size = >2kb
inserts in
EcoRI(5’)
XhoI(3’)
Grow in XL1 Blue MRF’ cells
Protocol book available from Stratagene
Constructed by Douglas
LaCount
In lambda-unizap (Stratagene)
4 x 1010 pfu/ml
average insert size = >2kb
inserts in
EcoRI(5’)
XhoI(3’)
Grow in XL1 Blue MRF’ cells
Protocol book available from Stratagene
Constructed by Douglas
LaCount