0.1 M Acetate, pH 4, 0.5 M NaCl

(used to wash CNBr-Sepharose-coupled ligand)

Kent Hill

3-10-00

A. 0.2 M NaOAc · 3 H₂O = 13.6 g in 500 ml

B. 0.2 M Acetic Acid = 5.78 ml glacial acetic acid in 500 ml H₂O

C. Titrate B with A to give final pH = 4.0

C. Take 500 ml C and add 29.22 g NaCl

E. QS 1 ltr

(Note: pH may drop to about 3.7 upon dilution (step E). This is ok for CNBr column washing. If desired, you can bring pH back up to 4 with 0.1 M NaOAc, and then add more NaCl as necessary to give final conc of 0.5 M NaCl.)