Department of Biochemistry
Iowa City, IA 52242-1109 USA
fax: (319) 335-9570
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Miles Pufall, PhD
Carver College of Medcine
University of Iowa
51 Newton Rd, 4-430 BSB
Iowa City, IA 52242
Office: 4-430 BSB
Phone: (319) 384-1820
Lab Phone: (319) 384-1821
Fax: (319) 335-9570
My overarching interest is in how proteins are regulated, and in particular that they are not simply turned on or off by single signals, but are modulated in response to multiple inputs to meet the needs of the cell at the time. In my work, I am studying how different signals, such as post-translational modifications, ligands, and association with other biological macromolecules including DNA, change the structure and dynamics of proteins of the glucocorticoid receptor, a steroid activated transcription factor that is one of the primary targets in the treatment of leukemias and lymphomas.
Garvie, Colin W., Pufall, Miles A., Graves, Barbara J., Wolberger, Cynthia. Structural analysis of the autoinhibition of Ets-1 and its role in protein partnerships., J. Biol. Chem., Vol. 277(47), 45529-36., Nov. 22, 2002. Download pdf reprint
Lee, Gregory M., Donaldson, Logan W., Pufall, Miles A., Kang, Hyun-Seo., Pot, Isabelle., Graves, Barbara J., McIntosh, Lawrence P., The structural and dynamic basis of Ets-1 DNA binding autoinhibition., J. Biol. Chem., Vol. 280(8), 7088-99., Feb. 25, 2005. Download pdf reprint
Pufall, Miles A., Lee, Gregory M., Nelson, Mary L., Kang, Hyun-seo., Velyvis, Algirdas, Kay, Lewis E., McIntosh, Lawrence P., Graves, Barbara J., Variable allosteric regulation of Ets-1 DNA binding by a multiply phosphorylated, unstructured region., Science., Vol. 309(5731), 142-145., July 1, 2005. Download pdf reprint
Pufall, Miles A.*, Lee, Greg M.*, Meeker, Charles A., Kang, Hyun-Seo, Graves, Barbara J., McIntosh Lawrence P., The affinity of Ets-1 for DNA is modulated by phosphorylation through transient interactions of an unstructured region., J. Mol. Biol., Vol. 382(4), 1014-30., Oct. 17, 2008. Download pdf reprint
Pufall, Miles A.*, Meijsing, Sebastiaan H.*, So, Alex Y., Bates, Darren L., Chen, Lin., Yamamoto, Keith R., DNA-binding site sequence directs glucocorticoid receptor structure and activity., Science., Vol. 324(5925), 407-410., April 17, 2009. Download pdf reprint
* Authors contributed equally to the work.
Holden Comprehensive Cancer Center
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